HomePage >> Journals >> Journal of Modern Agriculture

Journal of Modern Agriculture

ISSN Print:2169-592X

ISSN Online:2169-5938

Email:jma@ivypub.org

Website: http://www.ivypub.org/jma

  0
  0

Paper Infomation

Studies of a Cercospora Nicotianae Strain on Tobacco in China

Full Text(PDF, 5621KB)

Author: Fangfang Li, Wei Liu, Na Sun, Fenglong Wang, Lili Shen

Abstract: A tobacco leaf spot disease first prevalent in Shandong province of China was investigated according to Koch’s postulates. Fungal colonies on potato dextrose agar (PDA) were grey and velvet-like. The mycelium was initially colourless, but gradually turned dark brown and was septate. No spores were produced on PDA but the cultured mycelium was pathogenic to tobacco and produced reverse stick-shaped conidia with 3-20 diaphragms and its length was 256–806 µm × 4.1–19.5 µm. The conidiophore was straight, with 1–5 diaphragms and its length was 88–627 µm × 10.6–20.5 µm. Production of conidia was induced by adding tobacco leaf juice to oat agar culture and incubating in darkness at 27℃. Based on the morphological characteristics and ribosomal DNA-internal transcribed spacer (rDNA ITS) sequence analysis, the fungus was identified as Cercospora nicotianae (KM485926.1). The DNA (G+C) mol % was 51.86%, and fatty acid composition in cells was also examined. In bioassay, bordeaux, carbendazim and metalaxyl mancozeb showed good control against the pathogen.

Keywords: Cercospora Nicotianae; Identification; Control; Tobacco Frog Eye

References:

[1] Alasoadura SO, Fajola AO. Studies on the ‘frog eye’ disease of tobacco (Nicotiana tabacum L.) in Nigeria.Mycopathologia et Mycologia Applicate 42(1970):177-185

[2] Anjaneyulu C, Nagarajan k, Reddy TSN, Ramji L. Reaction of tobacco germplasm and Nicotiana species to frogeye spot (Cercospora nicotianae Ell. & Ev.) disease of tobacco. Tobacco Research 11(1985):105-109

[3] Fan JH, Chen HR. The research on the morphology and physiology of the pathogen of the frog eye. Journal of Yunnan Agricultural University 9(1994):179- 182

[4] Guo YL, Liu XJ. Flora fungorum sinicorum Cercospora. Beijing: Science Press (2005):1-373

[5] Guo YL. The relationships of taxonomy of Cercospora and its hoster. Journal of Jilin Agricultural University 20(1998):62-63

[6] He KJ, Huang SY, Pi P, Tang RY, Zhang YQ. Study on the biological characteristics and host range of the pathogen of the tobacco frog eye. Journal of Hunan Agricultural University 22(1996):170-173

[7] Landeweert R, Leeflang P, Kuyper TW, Hoffland E, Rosling A, Wernars K, Smit E. Molecular identification of ectomycorrhizal mycelium in soil horizons. Applied and Environmental Microbiology 69(2003):327-333

[8] Liu AM, He LQ. The correlation analysis of tobacco frog eye disease and meteorological factors. Hunan Agriculture Sciences (2000):29-31

[9] Pululu G, Corbaz R. Physalis angulata L., an important host of Cercospora nicotianae EII. And Ev. In Zaire. Annales du Tabacco, Section 2, 22(1989):89-94

[10] Shamarao J, Hundekar AR. Management strategies against frog eye leaf spot pathogen (Cercospora nicotianae) in India. Indian Phytopathology 63(2010):63-65

[11] Stavely JR, Nimmo JA. Effects of temperature upon growth and sporulation of Cercospora nicotianae. Phytopathology 59(1969):496-498

[12] White TJ, Bruns TD, Lee S, Taylor J. Analysis of phytogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. InnisMA. PCR Protocols: A guide to methods and applications. Academic, New York (1990)15-22

[13] Zhai FY, Li H, Wang XT, Liu YJ. Notes on provincial new record species of Cercospora in China. Acta Agriculturae Boreali-occidentalis Sinica 22(2013):172-175

[14] Zhu XC, Wang YT, Wang ZF. Tobacco Diseases of China. China Agriculture Press (2001):80-85

Privacy Policy | Copyright © 2011-2024 Ivy Publisher. All Rights Reserved.

Contact: customer@ivypub.org