Analysis of Effect of High Numerical Aperture on the Resolution in STED Microscopy

HomePage >> Journals >> Biotechnology Frontier

Biotechnology Frontier

Biotechnology Frontier (Yearly) is an international comprehensive professional academic journal of Ivy Publisher, concerning the development of biotechnology technology. The main focus of the journal is the academic papers, comments and research review of latest improvement in the fields of Biotechnology technology, microorganism, medicine, agriculture & forestry, edible fungus, light food, environmental protection and related, aiming at... [More] Biotechnology Frontier (Yearly) is an international comprehensive professional academic journal of Ivy Publisher, concerning the development of biotechnology technology. The main focus of the journal is the academic papers, comments and research review of latest improvement in the fields of Biotechnology technology, microorganism, medicine, agriculture & forestry, edible fungus, light food, environmental protection and related, aiming at providing a good communication platform to transfer, share and discuss the theoretical and technical development of electrical theory development for professionals, scholars, researchers and administrative staffs in this field, reflecting the academic front level, promote academic change and foster the development of biotechnology technology.

The journal receives manuscripts written in Chinese or English. As for Chinese papers, the following items in English are indispensible parts of the paper: paper title, author(s), author(s)'affiliation(s), abstract and keywords. If this is the first time you contribute an article to the journal, please format your manuscript as per the sample paper and then submit it into the online submission system. Accepted papers will immediately appear online followed by printed hard copies by Ivy Publisher globally. Therefore, the contributions should not be related to secret. The author takes sole responsibility for his views.

ISSN Print:2327-0837

ISSN Online:2327-0888

Email:bf@ivypub.org

Website: http://www.ivypub.org/bf/

		0
		0

Navigation

News

All articles submitted to this journal are required to be tested by AMLC.

This journal is full-text indexed by Centre Agriculture Bioscience International (CABI), which is one of the world's three major agriculture literature database.

This journal is full-text indexed by CNKI which is the China's most authoritative academic resources database.

Paper Infomation

Analysis of Effect of High Numerical Aperture on the Resolution in STED Microscopy

Full Text(PDF, 762KB)

Author: Zhang Hong, Jihong Feng, Sen Zhang, Xinwei Gao

Abstract: In this paper we analyze the effect of high numerical aperture (NA) on the depletion beam’s intensity distribution in stimulated emission depletion (STED) microscopy. Based on the Debye vector diffraction integral formula, we analyze the STED depletion beam which passes through a vortex 0/2π phase plate and then through a high NA lens in the STED microscopy. The research shows that the full width half maximum (FWHM) of the depletion beam decreases when the NA increases; however, the FWHM remains unchanged when the NA varies from 1.15 to 1.30; on the contrary, it increases unexpectedly when NA is 1.35 and 1.4. These results suggest that the resolution in STED microscopy increases when the NA increases generally, but it is not true that the larger the NA is, the higher the resolution will preliminarily be.

Keywords: Stimulated Emission Depletion Microscopy; Resolution; Intensity Distribution; Numerical Aperture; Full Width Half Maximum

References:

[1] S. W. Hell, J. Wichmann. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy[J]. Opt.Lett., 1994, 19(11): 780-782.

[2] Huang Bo, Babcock Hazen, Zhuang Xiaowei. Breaking the diffraction barrier: super-resolution imaging of cells[J].Cell, 2010, 143(7): 1047-1058.

[3] Hao Xiang, Kuang Cuifang, Wang Tingting. Effects of polarization on the de-excitation dark focal spot in STED microscopy[J]. Journal Of Optics, 2010, 12(11).

[4] I. Testa, N. T. Urban, S. Jakobs, et al. Nanoscopy of Living Brain Slices with Low Light Levels[J]. Neuron, 2012, 75(6): 992-1000.

[5] S.W. Hell, Breaking Abbe’s barrier: diffraction-unlimited resolution in far-field microscopy[J]. Cytometry Part A, 2007, 71A(9): 742-742.

[6] G. Moneron, S. W. Hell. Two-photon excitation STED microscopy[J]. Opt.Express, 2009, 17(17): 14567-14573.

[7] Klar T A , Hell S W. Subdiffraction resolution in far-field fluorescence microscopy[J]. Opt Lett, 1999, 24(14) : 954-956.

[8] T. A. Klar, E. Engel, S. W. Hell. Breaking Abbe’s diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes [J]. Physical Review E, 2001, 64(6): 066613.

[9] V. Westphal, C. M. Blanca, M. Dyba, et al,Laser-diode-stimulated emission depletion microscopy[J]. Applied Physics Letters, 2003, 82(18): 3125-3127.

[10] V. Westphal, L. Kastrup, S. W. Hell. Lateral resolution of 28 nm (λ/25) in far-field fluorescence microscopy[J]. Appl. Phys, 2003, 77(4): 377-380.

[11] D. Wildanger, J. Buckers, V.Westphal, et al,A STED microscope aligned by design[J]. Optics Express, 2009, 17(18): 16100-16110.

[12] G.M. Lerman, U. Levy. Tight focusing of spatially variant vector optical fields with elliptical symmetry of linear polarization[J]. Opt. Lett., 2007, 32(15): 2194-2196.

[13] J. W. M. Chon, X. Gan, M. Gu. Splitting of the focal spot of a high numerical-aperture objective in free space[J]. Appl.Phys.Lett., 2002, 81(9): 1576-1578.

[14] Chen Baosuan, Pu Jixiong. Focusing of elliptically polarized vortex beams through a high numerical-aperture objective[J].Chinese Physics B, 2010, (7): 249-254.

[15] Hao Xiang, Kuang Cuifang, Wang Tingting, et al. Phase encoding for sharper focus of the azimuthally polarized beam [J]. Opt. Lett., 2010, 35( 23): 3928-3930.

[16] Kuang Cuifang, Hao Xiang, Liu Xu, et al. Formation of sub-half-wavelength focal spot with ultra long depth of focus [J]. Optics Communications, 2011, 284(7): 1766-1769.

[17] Xue Yi, Kuang Cuifang, Li Shuai., et al. Sharper fluorescent super-resolution spot generated by azimuthally polarized beam in STED microscopy [J]. Optics Express, 2012, 20(16) : 17653-17666.