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Biotechnology Frontier

Biotechnology Frontier (half-yearly) is an international comprehensive professional academic journal of Ivy Publisher, concerning the development of biotechnology technology. The main focus of the journal is the academic papers, comments and research review of latest improvement in the fields of Biotechnology technology, microorganism, medicine, agriculture & forestry, edible fungus, light food, environmental protection and related, aiming at... [More] Biotechnology Frontier (half-yearly) is an international comprehensive professional academic journal of Ivy Publisher, concerning the development of biotechnology technology. The main focus of the journal is the academic papers, comments and research review of latest improvement in the fields of Biotechnology technology, microorganism, medicine, agriculture & forestry, edible fungus, light food, environmental protection and related, aiming at providing a good communication platform to transfer, share and discuss the theoretical and technical development of electrical theory development for professionals, scholars, researchers and administrative staffs in this field, reflecting the academic front level, promote academic change and foster the development of biotechnology technology.

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ISSN Print:2327-0837

ISSN Online:2327-0888

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Paper Infomation

Analysis of Effect of High Numerical Aperture on the Resolution in STED Microscopy

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Author: Zhang Hong, Jihong Feng, Sen Zhang, Xinwei Gao

Abstract: In this paper we analyze the effect of high numerical aperture (NA) on the depletion beam’s intensity distribution in stimulated emission depletion (STED) microscopy. Based on the Debye vector diffraction integral formula, we analyze the STED depletion beam which passes through a vortex 0/2π phase plate and then through a high NA lens in the STED microscopy. The research shows that the full width half maximum (FWHM) of the depletion beam decreases when the NA increases; however, the FWHM remains unchanged when the NA varies from 1.15 to 1.30; on the contrary, it increases unexpectedly when NA is 1.35 and 1.4. These results suggest that the resolution in STED microscopy increases when the NA increases generally, but it is not true that the larger the NA is, the higher the resolution will preliminarily be.

Keywords: Stimulated Emission Depletion Microscopy; Resolution; Intensity Distribution; Numerical Aperture; Full Width Half Maximum

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