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Ginsenoside Rg3 Increases the Sensitivity of Ovarian Cancer Cells to Cisplatin and Its Mechanism
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Author: Yan Sang, Qin Zheng
Abstract: To investigate the effect and mechanism of ginsenoside Rg3 on proliferation and cisplatin sensitization of ovarian cancer cells SKOV3. Methods: The cell activity of ginsenoside Rg3 and cisplatin on ovarian cancer cells was measured by CCK-8 method. Cell morphology was observed by inverted microscope. Cell migration was investigated by scratch test. To investigate the effect of ginsenoside Rg3 on the cloning ability of SKOV3 cells. Western blot assay was used to investigate the effect of ginsenoside Rg3 on the expression level of PI3K/Akt/mTOR signaling pathway in SKOV3 cells. Results: Ginsenoside Rg3 inhibited SKOV3 cells in a time-dependent and dose-dependent manner. Ginsenoside Rg3 increased SKOV3 cells' sensitivity to cisplatin. When the cells were examined under microscope, it was found that compared with DDP alone, the cells became round and apoptotic cells increased. The clonal formation data showed that with the increase of cisplatin concentration, the number of clonal formation of SKOV3 cells was significantly reduced, and the addition of ginsenoside Rg3 could further inhibit the formation. Scratch experiments showed that ginsenoside Rg3 could further inhibit the migration of ovarian cancer cells under DDP. Western blot analysis showed that ginsenoside Rg3 could reduce the protein expression level of PI3K/Akt/mTOR signaling pathway. Conclusion: Ginsenoside Rg3 can control the proliferation and progression of SKOV3 cells, promote cell sensitivity to cisplatin, inhibit the expression of PI3K/Akt/mTOR signaling pathway, and increase the sensitivity to cisplatin by inhibiting the expression of this signaling pathway.
Keywords: Ovarian Cancer, Cisplatin, Ginsenoside Rg3, PI3K/Akt/mTOR Signaling Pathway
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