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Biotechnology Frontier

Biotechnology Frontier (half-yearly) is an international comprehensive professional academic journal of Ivy Publisher, concerning the development of biotechnology technology. The main focus of the journal is the academic papers, comments and research review of latest improvement in the fields of Biotechnology technology, microorganism, medicine, agriculture & forestry, edible fungus, light food, environmental protection and related, aiming at... [More] Biotechnology Frontier (half-yearly) is an international comprehensive professional academic journal of Ivy Publisher, concerning the development of biotechnology technology. The main focus of the journal is the academic papers, comments and research review of latest improvement in the fields of Biotechnology technology, microorganism, medicine, agriculture & forestry, edible fungus, light food, environmental protection and related, aiming at providing a good communication platform to transfer, share and discuss the theoretical and technical development of electrical theory development for professionals, scholars, researchers and administrative staffs in this field, reflecting the academic front level, promote academic change and foster the development of biotechnology technology.

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ISSN Print:2327-0837

ISSN Online:2327-0888


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Paper Infomation

Cloning And Characterization Of A Geranylgeranyl Diphosphate Synthase Gene From Taxus Chinensis

Full Text(PDF, 7870KB)

Author: Junhao Shen, Yangyang Qiao, Yongqin Chen, Jungui Dai, Qiqi Chen, Zhifan Yang, Xuzhi Ruan, Ronghua Wei

Abstract: Taxol (Paclitaxel) is a diterpene from Taxus species and has been used in treatment of various kinds of cancers. Geranylgeranyl diphosphate synthase (GGPPS) catalyzes the formation of geranylgeranyl diphosphate (GGPP, the common precursor for diterpenes and plays a key role in taxol biosynthesis. Here we report a functional GGPPS gene from Taxus chinensis (designated TcGGPPS). TcGGPPS is an intron free gene and has a 1,182-bp open reading frame encoding a polypeptide of 393 amino acid residues with a calculated molecular mass of 42.63 kDa and an isoelectric point of 5.58. The catalytic activity of TcGGPPS for production of GGPP was verified by a color enhancement assay in the Escherichia coli cells harboring plasmid pAC-BETA. Multiple sequence alignment indicates that TcGGPPS is a little different in sequence from the functional GGPPS genes from other Taxus species such as T. canadensis, T. media and T. wallichiana, which are almost identical to each other. Protein structure prediction by using bioinformatics reveals that TcGGPPS consists of 52.2% α-helix, 10.9% extended strand, 8.4% β-turn and 28.5% random coil, and has a three-dimensional structure highly similar to the structurally known Sinapis alba GGPPS. In silicon predictions also demonstrate that TcGGPPS has a plastid-targeting peptide at the N-terminus, suggesting it is responsible for the synthesis of GGPP in plastids.

Keywords: Taxus chinensis; Geranylgeranyl Diphosphate Synthase; Gene Cloning; Taxol Biosynthesis


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