Paper Infomation
Detection of Carbofuran Pesticide Using an Enzyme Biosensor Based on MWNTs-PDDA Modified Glassy Carbon Electrode
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Author: Shuping Zhang, Ying Liu, Xingkai Ye, Song Qu, Jie Ma, Zhenran Tian, Lei Cui
Abstract: This paper presents the construction of an amperometric biosensor for the highly sensitive detection of the carbamate insecticide carbofuran, based on the inhibition of acetylcholinesterase (AChE). Using an enzyme biosensor based on multi-walled carbon nanotubes (MWNTs) and poly diallyldimethylammonium chloride (PDDA) modified glassy carbon electrode (GCE), then immobilizing the Acetylcholinesterase (AChE) to the modified GCE with layer-by-layer (LBL) technique directly. Detect the inhibition of Aches with the amperometric i-t response. The pesticide concentration is correlated to the percentage inhibition of enzyme activity. Then detect the carbofuran by the enzyme electrode with 0.01U activity value and it shows a good linearity in the range from 10-12 g/L to 10-6 g/L between the inhibition rates and -lg[carbofuran], and the detection limit is 10-12 g/L.
Keywords: Enzyme Biosensor; AChE; Layer-by-layer (LBL); Carbofuran
References:
[1] WHO (1986) Environmental Health Criteria. World Health Organization, Geneva
[2] G.W. Vanloon, J. Duffy, Environmental Chemistry—A global Perspective, University Press, Oxford, 2001
[3] S. Okazaki, H. Nakagawa, K. Fukuda, S. Asakura, H. Kiuchi, T. Shigemori, S. Takahashi, Sens. Actuators B 66 (2000) 131
[4] J.M. Soriano, B. Jim´enez, G. Font, J.C. Molt´o, Crit. Rev. Anal. Chem. 31 (2001) 19
[5] H. Jing, A. Amirav, Anal. Chem. 1997, 69, 1426
[6] G. S. Nunes, M. L. Ribeiro, L. Polese, D. Barcelo, J. Chromatogr. A 1998, 795, 43
[7] Vakurov A, Simpson CE, Daly CL, Gibson TD, Millner PA (2004) Biosens Bioelectron 20:1118-1125
[8] Law KA, Higson SPJ (2005) Biosens Bioelectron 20:1914-1924
[9] Bucur B, Fournier D, Danet A, Marty J-L (2006) Anal Chim Acta 562:115-121
[10] Andreescu S, Marty J-L (2006) Biomol Eng 23:1-15
[11] D. Voet, J.G. Voet, Biochemistry, Wiley, New York, 1990, p. 1168
[12] Andreescu S, Noguer T, Magearu V, Marty J-L (2002) Talanta 57:169-176
[13] Schulze H, Vorlova S, Villatte F, Bachmann TT, Schmid RD (2003) Biosens Bioelectron 18:201-209
[14] De Oliveira Marques PRB, Nunes GS, dos Santos TCR, Andreescu S, Marty J-L (2004) Biosens Bioelectron 20:825-832
[15] G. Liu, Y. Lin, Electrochem. Commun. J. 8 (2006) 251
[16] H. Zhao, J. Huang, Anal. Biochem. J. 350 (2006) 138