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Scientific Journal of Frontier Chemical Development

ISSN Print:2167-163X

ISSN Online:2167-1648


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Paper Infomation

Detection of Carbamate Pesticides based on MWNTs-chitosan Modified Carbon Nanotube Electrode Biosensor with Immobilized Acetylcholinesterase

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Author: Xingkai Ye, Shuping Zhang

Abstract: A stable and highly sensitive enzyme biosensor based on MWNTs-chitosan modified glassy carbon electrode (GCE) with efficient immobilization of acetylcholinesterase (AChE) has been devised for rapid determination of carbamate pesticides especially methomyl, aldicarb, carbaryl and fenobucarb. AChE was immobilized via layer-by-layer self-assembly modification technique. The study indicated that the inhibition of carbamate pesticides on the AChE activity was proportional to concentration of carbamate pesticides in the 10-10 g·L-1 to 10-3 g·L-1 concentration range and the limitation of detecting methomyl is up to 10-12 g·L-1 and others is 10-11 g∙L-1. So the enzyme biosensor may be an alternative new tool for analyzing pesticide remains in environment or food.

Keywords: Enzyme Biosensor; AChE; LBL; CS; Carbamate Pesticides; Methomyl


[1] Flores F., Artigas J., Marty J.L., etc. Development of an EnFET for the detection of organophosphorous and carbamate insecticides. Anal Bioanal Chem, 2003, 376, 476-480

[2] Muzzarelli R.A.A., Muzzarelli C. Chitosan Chemistry: Relevance to the Biomedical Sciences. Adv Polymer Sci, 2005, 186, 151-209

[3] Davis J.J., Coles R.J., Hill A.O., etc. Protein electrochemistry at carbon nanotube electrodes. Electroanal Chem, 1997, 440, 279-282

[4] Zeng J; Wei W., Liu X., etc. A simple method to fabricate a Prussian Blue nanoparticles/carbon nanotubes/poly (1, 2-diaminobenzene) based glucose biosensor. Microchimca Acta, 2008, 160, 261-267

[5] Iijima S. Helical microtubules of graphitic carbon. Nature, 1991, 354, 56-58.

[6] Espinosa M, Atanasov P, Wilkins E. Development of a Disposable Organophosphate Biosensor. Electroanalysis, 1999, 11, 1055-1062

[7] E. Suprun, G. Evtugyn, H. Budnikov, etc. Acetylcholinesterase sensor based on screen-printed carbon electrode modified with prussian blue. Anal Bioanal Chem, 2005, 383, 597- 604

[8] Dan Du, Xi Huang, Jie Cai, etc. An amperometric acetylthiocholine sensor based on immobilization of acetylcholinesterase on a multiwall carbon nanotube–cross-linked chitosan composite. Anal Bioanal Chem, 2007, 387, 1059-1065

[9] Jie Cai, Dan Du. A disposable sensor based on immobilization of acetylcholinesterase to multiwall carbon nanotube modifiedscreen-printed electrode for determination of carbaryl. Appl Electrochem, 2008, 38, 1217-1222

[10] Zhou, Z.X., Guo, X.M., Wang, L.G., etc. The study of Ache bio-sensor. Farm chemicals, 2008, 47, 577-579

[11] Banks, C.E., Davies, T.J., Wildgoose, G.G., etc. Electrocatalysis at graphite and carbon nanotube modified electrodes:edge-plane sites and tube ends are the reactive sites. Chem. Commun, 2005, 7, 829-841

[12] Zhang, S.P., Shan, L.G., Tian, Z.R., etc. Study of enzyme biosensor based on carbon nanotubes modified electrode for detection of pesticides residue. Chinese Chem. Lett, 2008, 19, 592-594

[13] Boyer, P.D., Lordy, H.A., Murback, K. The Enzymes, vol. 7, second ed., Academic Press, New York, 1963

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