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Scientific Journal of Frontier Chemical Development

ISSN Print:2167-163X

ISSN Online:2167-1648

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Determination of Baicalin by Enhanced Fluorimetry and Anti-Oxidation Effect of Baicalin

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Author: Lijuan Du, Yan Hu, Xiaoming Li, Manxiu Li

Abstract: The interaction between compound of Tween 80/benzene/n-butyl alcohol/water O/W microemulsion medium and β-CD were studied by spectrofluorimetry. Based on the significant enhancement of fluorescence intensity of analysed in inclusion complex,a spectrofluorimetry method with high sensitivity and selectivity was developed for the determination of Baicalin in aqueous solution. The linear range of the method was 5.0×10-6mol/L—4.0×10-5mol/L with a detection limit of 1.5×10-6mol/L, and the relative standard deviation was 0.98%.The recoveries of deltamethrin are 99.2% -101.0%. The results also showed that Baicalin have good antioxidant activity.

Keywords: Baicalin; β-CD; O/W Microemulsion; Enhanced Fluorimetry; Antioxidant Activity.

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